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Spin Column Viral RNA Extraction Kit

    Buy cheap Spin Column Viral RNA Extraction Kit from wholesalers
     
    Buy cheap Spin Column Viral RNA Extraction Kit from wholesalers
    • Buy cheap Spin Column Viral RNA Extraction Kit from wholesalers
    • Buy cheap Spin Column Viral RNA Extraction Kit from wholesalers
    • Buy cheap Spin Column Viral RNA Extraction Kit from wholesalers
    • Buy cheap Spin Column Viral RNA Extraction Kit from wholesalers
    • Buy cheap Spin Column Viral RNA Extraction Kit from wholesalers
    • Buy cheap Spin Column Viral RNA Extraction Kit from wholesalers

    Spin Column Viral RNA Extraction Kit

    Ask Lasest Price
    Brand Name : Renji Medical
    Model Number : RJ-B
    Certification : CE, SGS, MSDS, YBTC, MA
    Price : Negotiable
    Payment Terms : T/T. 50% deposit before production, balance be paid before shipping.
    Supply Ability : 500K pieces per day
    Delivery Time : 5-8 working days
    • Product Details
    • Company Profile

    Spin Column Viral RNA Extraction Kit

    Viral RNA Extraction Kit (Spin Column) used for nucleic acid

    extraction with high nucleic acid purity in subsequent experiments

    (DNA/RNA)


    PRODUCT NAME
    Viral RNA&DNA Extraction Kit
    METHOD
    Spin Column Method
    Spin Column Viral DNA/RNA Extraction Kit utilizes a unique lysis buffer to lyse
    virus and release DNA / RNA. The released DNA / RNA is effectively purified after
    specifically binding to a silica-based spin column. It is suitable for isolating viral
    DNA/RNA from up to 200 μl of plasma, serum, whole blood, tissue homogenate,
    cell-free body fluid, nasopharyngeal or oropharyngeal aspirate/wash, bronchoalveolar
    lavage fluid (BALF), tracheal aspirate, sputum, nasopharyngeal or oropharyngeal swab,
    and animal cell culture supernatant. The isolated DNA/RNA with high purity can be
    applied in PCR, RT-PCR, qPCR, qRT-PCR, etc.

    Spin columns are designed for fast, simple, and efficient extraction of DNA and RNA
    from biological samples such as bacteria, viruses, yeast, cultured cells, fresh and
    dried blood, urine, buccal swabs, animal tissue. They can also be used for DNA and
    RNA PCR product cleanup and nucleic acid gel extraction.
    Using classic silica membrane adsorption technology and a unique buffer system,
    Virus DNA/RNA extraction Kits (spin column) can extract DNA/RNA from blood,
    tissue, organs, environmental sample, saliva, and nasal sample, and maximum
    remove impurities protein and other organic compound impurities. The extracted
    virus DNA/RNA can be directly used for downstream applications such as PCR,
    RT-PCR, qPCR, and qRT-PCR experiment.
    Viral Nucleic Acid Isolation Kit (Silica-Based Spin Column) is specially designed for
    efficient purification of viral RNA and viral DNA from cell-free samples such as
    serum, plasma, urine, body fluids, and the supernatant of virally infected cell cultures.
    The sample is lysed by incubation with a Lysis Solution. The Lysis Solution inactivates
    RNases, ensuring the protection of viral nucleic acids against degradation. The lysed
    sample is transferred to a spin column where released viral nucleic acids immediately
    bind to the silica-based filter in the presence of Binding Buffer. The remaining
    contaminants are removed during three wash steps using Wash Buffer, whereas
    pure nucleic acids remain bound to the membrane. Pure viral nucleic acids are
    released from the spin column filter using Elution Buffer. The purified nucleic
    acids are ready-for-use in subsequent downstream applications of molecular biology.
    Spin Column Viral RNA Extraction KitSpin Column Viral RNA Extraction Kit
    APPLICATIONS
    • Extraction of viral RNA and DNA and microbial DNA from clinical samples
    • Clinical testing applications
    • Suitable for DNA/RNA extraction from blood, animal tissue, environmental
    sample, saliva, nasal liquid, and swab sample.
    • Suitable Sample Main Components: Stool, Swab, Sputum, Serum, and Plasma

    FEATURES


    Viral RNA&DNA Extraction Kit (Spin column) The kit utilizes silica-based membrane

    technology in the form of a convenient spin column. Viral nucleic acids from lysed

    samples bind to the column membrane while impurities are effectively removed

    during subsequent washing and centrifugation steps. Ready-to-use nucleic acids

    are eluted from the column. The kit allows simplified isolation of viral RNA from

    cell-free body fluids with a fast spin-column format. No phenol/chloroform extraction

    is required. Viral RNA binds specifically to the silica membrane while contaminants

    are removed in the flow-through. PCR inhibitors such as divalent cations and proteins

    are completely removed in two efficient wash steps, leaving pure viral RNA to be

    eluted in RNase-free water. Purified RNA is ready to use in RT-PCR, Northern

    blotting or other downstream applications.


    • The operation process is simple, quick, and non-toxic
    • High purity DNA/RNA, stable quality
    Benefits
    • Comprehensive sample type
      Highly-pure viral RNA extraction from various clinical samples such as serum,

    blood samples, CSF (Cerebrospinal Fluid), urine, swab, etc.

    • Poly(A) powder
      Maximized RNA degradation protection and binding efficiency
    • High binding capacity
      Utilization of Silica Magnetic Particles developed and produced by proprietary

    technology

    • Convenience
      Ethanol already added to the Wash Buffer

    Application

    • cDNA synthesis
    • RT-PCR
    • Quantative real-time RT-PCR
    • poly A+ RNA selection
    • Northern blot analysis
    • Pathogen detection
    Spin Column Viral RNA Extraction KitSpin Column Viral RNA Extraction Kit
    Spin Column Viral RNA Extraction Kit

    PACKAGE SPECIFICATION
    50 tests/kit
    INTENDED USE
    This kit is used to extract DNA/RNA from plasma, serum, animal tissues, body fluids
    and other samples. The sample is processed by the lysis solution to release DNA/RNA.
    At the same time, a special polymer membrane material is used to selectively adsorb
    viral DNA/RNA, and then after washing and elution operations, high-purity viral
    DNA/RNA can be obtained. The nucleic acid extracted by this kit can be used for
    Real-time PCR, Nothern Blot, Blotting Hybridization, cDNA Library Construction.
    The kit can be used for both research purposes and in vitro diagnosis (IVD) and
    is validated for COVID-19 diagnosis.
    COMPONENTS OF THE KIT
    No.Reagent NameSpec. & Qty
    1Lysis Solution15mlx 1 Bottle
    2Wash Buffer I8ml×1 Bottle (add 24ml absolute ethanol before the first use)
    3Wash BufferⅡ8ml×1 Bottle (add 24ml absolute ethanol before the first use)
    4Elution Solution3mlx 1 Bottle
    5Adsorption Column50×1 Pack
    6Collection Tube
    50×1 Pack

    STORAGEAND STABILITY
    The kit should be stored at room temperature 5-25℃. The kit is valid for 12 months.

    【Self Provide Materials】

    Sterilized 1.5ml centrifuge tube, Sterilized tips of various specifications, centrifuge,

    absolute ethanol, vortex mixer, etc.

    SPECIMEN REQUIREMENTS
    1. The virus in liquid samples such as whole blood, plasma, serum, ascites, oral fluid
    can be directly extracted.
    2. The virus in animal and plant tissues needs to add an appropriate amount of saline
    or PBS to the sample, grind it thoroughly, centrifuge at 12000rpm for 5-10 minutes,
    and take the Supernatant for use
    SPECIMEN EXTRATION OPERATION
    1. Add 300ul of the processed sample to a 1.5ml clean centrifuge tube (if less than
    300ul, please make up to 300ul with PBS or saline), and then add 300ul of Lysis
    Solution. Cap the tube and vortex for 30 seconds.
    2. Incubate it at room temperature for 10 minutes.
    3. Add 200ul of absolute ethanol to the centrifuge tube, cap the tube, and vortex for
    15 seconds. Centrifuge briefly to collect the solution on the tube wall and tube cap.
    4. Put the Adsorption Column into the Collection Tube, transfer the above mixed
    solution to the Adsorption Column, centrifuge at 8000rpm for 1 minute, and discard
    the solution in the Collection Tube.
    5. Add 600ul of Washing Buffer I to the Adsorption Column (confirm that absolute
    ethanol was added to Washing Solution I before use), centrifuge at 8000rpm for
    1 minute, and discard the solution in the Collection Tube.
    6. Add 600ul of Washing BufferⅡto the Adsorption Column (confirm that absolute
    ethanol was added to Washing SolutionⅡbefore use), centrifuge at 8000rpm for l
    minute, and discard the solution in the Collection Tube.
    7. Put the Adsorption Column back into the collection tube and centrifuge at 8000rpm
    for 2 minutes, discard the solution in the Collection Tube.
    8. Transfer the Adsorption Column to a new 1.5ml centrifuge tube, add 50pl of
    Elution Solution to the Adsorption Column and incubate at room temperature
    for 2 minutes.
    9. Centrifuge at 8000rpm for 1 minute and discard the Adsorption Column.
    The solution in the 1.5ml centrifuge tube contains DNA/RNA. The extracted DNA/RNA
    can be directly used in Real-time PCR, Nothern Blot, Blotting Hybridization, cDNA
    Library Construction and other experiments. If you do not use it immediately,
    please store it at-20℃.
    PRODUCT PERFORMANCE INDEX
    1. The purity:The ratio of absorbance OD260/OD280 is between 1.8-2.2.
    2. The precision:The coefficient of variation (CV) of the RNA/DNA content is≤5%.
    【SAMPLE REQUIREMENTS
    • Store at 4°C for no more than 72 hours; at -70°C for long term storage
    • Avoid repeated freezing and thawing
    • Swab samples should only be collected with synthetic tip swabs with aluminum or
    plastic shafts.
    PRECAUTIONS
    1. Please read this kit operating instructions carefully before use, and operate carefully
    as required.
    2. The virus has a strong infection ability, and various defense measures must be
    prepared before operation.
    3. Wear clean gloves and masks during operation.
    4. Use RNase/DNase-free tips and 1.5ml centrifuge tubes.
    5. All centrifugation steps are performed at room temperature (5-25℃).
    6. Frozen samples need to be thawed to room temperature.
    7. Need to bring your own absolute ethanol and 75% ethanol,
    8. Do not use products beyond the expiration date; do not mix reagents of different
    lot numbers.

    INDEX OF SYMBOL


    EXPORTER

    Magnus International Limited


    F12, New City International Mansion A, 234 Huapao Ave.

    Liuyang, Hunan Province 410300 China


    Contact: Goodwellmedical@gmail.com


    AUTHORIZED REPRESENTATIVE


    Lotus NL B.V.

    Koningin Julianaplein 10, 1e Verd, 2595AA, The Hague, Netherlands.


    FACTORY VIEW


    Sterile Pipette Tips, Filtered, DNAse and RNAse Free, Autoclavable - 200 µl 3


    Sterile Pipette Tips, Filtered, DNAse and RNAse Free, Autoclavable - 200 µl 4


    FACTORY WORKSHOP


    Sterile Pipette Tips, Filtered, DNAse and RNAse Free, Autoclavable - 200 µl 5


    Sterile Pipette Tips, Filtered, DNAse and RNAse Free, Autoclavable - 200 µl 6


    PRODUCT AND STANDARDS


    Sterile Pipette Tips, Filtered, DNAse and RNAse Free, Autoclavable - 200 µl 7


    【CERTIFICATIONS】


    Sterile Pipette Tips, Filtered, DNAse and RNAse Free, Autoclavable - 200 µl 9


    Sterile Pipette Tips, Filtered, DNAse and RNAse Free, Autoclavable - 200 µl 10


    【FAQ

    1. Q: How about your healthcare and medical product quality?
    A: Quality is our top priority. We have a professional quality control team to ensure the
    quality with a high standard and regulated with the pro certifications. The QC team is
    responsible for quality checking in each process and inspects every batch of our production
    and ships them only after QC approval.
    2. Q: What is your order MOQ?
    A: Please contact customer service, and the price is negotiable for a large quantity.
    3. Q: How about your product packing for shipment or mailing?
    A: We prepared a well neutral package for our products. We could also make OEM packages,
    OEM/ODM Custom Package/Multi-packing package/Cartons with well protective wrapping.
    4. Q: What level of your product price?
    A: We are the factory and direct manufacturer and offer competitive prices. We also offer
    volume pricing with more benefits depending on your order quantity level. Please let us know
    your ordering volume while you make the inquiry to us to help you obtain better benefits.
    5. Q: What're the price terms?
    A: The price is based on the FOB of Hunan, China with an exchange rate of RMB6.40 for
    USD$1.00. If the exchange rate floating sharply lower than 6.3 or higher than 6.5, we should
    adjust the price with the real-time exchange rate.
    6. Q: What're the payment terms?
    A: We accept T/T wire transferring, PayPal, Western Union, etc. 50% down payment after
    signing the contract and rest 50% before shipping confirmation.
    7. Q: How could I get some samples?
    A: We are able to deduct your samples expense once your orders are confirmed with us.
    The shipping fee will be calculated with the sample volume that you demand.
    8. Q: Could you accept OEM orders?
    A: Yes, we could. We have professional designers who can design the logo or artwork and offer
    best solution & styles for your OEM product choices.
    9. Q: How long will the shipping or express take?
    A: (1) For the international express such as DHL, UPS, FedEx, TNT, or EMS, etc., it usually
    takes 5-7 working days to your location or destination.
    (2) If shipping with containers, they will take about 30-60 days to process depending on
    the various ports or door locations.
    (3) We will arrange the delivery or shipping of your products within 1-3 days after full
    payment has been confirmed. We may not ship your products out until your payments arrive
    to our bank account successfully.
    10. Q: How do you deliver the goods to us?
    A: If the order of small volume package, we could deliver by courier such as DHL, UPS, FedEx,
    TNT, EMS. For the large volume order, we could ship with bulk cargo containers by sea.
    We will provide you with cost-saving recommendations for your product shipping.
    11. Q: What is the warranty time.
    A: One year for each product.
    Sterile Pipette Tips, Filtered, DNAse and RNAse Free, Autoclavable - 200 µl 8
    SHIPPING
    Sterile Pipette Tips, Filtered, DNAse and RNAse Free, Autoclavable - 200 µl 11
    We are the trusted healthcare and medical supplies from China.
    Be free to contact us at any time!

    You are very welcome to email us today at:
    Goodwellmedical@gmail.com for the inquiry!

    We are excited to build a reliable and long-term business with you
    at our best!
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