Viral RNA Extraction Kit (Spin Column) used for nucleic acid
extraction with high nucleic acid purity in subsequent experiments
(DNA/RNA)
【PRODUCT NAME】
Viral RNA&DNA Extraction Kit
【METHOD】
Spin Column Method
Spin Column Viral DNA/RNA Extraction Kit utilizes a unique lysis
buffer to lyse
virus and release DNA / RNA. The released DNA / RNA is effectively
purified after
specifically binding to a silica-based spin column. It is suitable
for isolating viral
DNA/RNA from up to 200 μl of plasma, serum, whole blood, tissue
homogenate,
cell-free body fluid, nasopharyngeal or oropharyngeal
aspirate/wash, bronchoalveolar
lavage fluid (BALF), tracheal aspirate, sputum, nasopharyngeal or
oropharyngeal swab,
and animal cell culture supernatant. The isolated DNA/RNA with high
purity can be
applied in PCR, RT-PCR, qPCR, qRT-PCR, etc.
Spin columns are designed for fast, simple, and efficient
extraction of DNA and RNA
from biological samples such as bacteria, viruses, yeast, cultured
cells, fresh and
dried blood, urine, buccal swabs, animal tissue. They can also be
used for DNA and
RNA PCR product cleanup and nucleic acid gel extraction.
Using classic silica membrane adsorption technology and a unique
buffer system,
Virus DNA/RNA extraction Kits (spin column) can extract DNA/RNA
from blood,
tissue, organs, environmental sample, saliva, and nasal sample, and
maximum
remove impurities protein and other organic compound impurities.
The extracted
virus DNA/RNA can be directly used for downstream applications such
as PCR,
RT-PCR, qPCR, and qRT-PCR experiment.
Viral Nucleic Acid Isolation Kit (Silica-Based Spin Column) is specially designed for
efficient purification of viral RNA and viral DNA from cell-free
samples such as
serum, plasma, urine, body fluids, and the supernatant of virally
infected cell cultures.
The sample is lysed by incubation with a Lysis Solution. The Lysis
Solution inactivates
RNases, ensuring the protection of viral nucleic acids against
degradation. The lysed
sample is transferred to a spin column where released viral nucleic
acids immediately
bind to the silica-based filter in the presence of Binding Buffer.
The remaining
contaminants are removed during three wash steps using Wash Buffer,
whereas
pure nucleic acids remain bound to the membrane. Pure viral nucleic
acids are
released from the spin column filter using Elution Buffer. The
purified nucleic
acids are ready-for-use in subsequent downstream applications of
molecular biology.
【APPLICATIONS】
sample, saliva, nasal liquid, and swab sample.
- Suitable Sample Main Components: Stool, Swab, Sputum, Serum, and
Plasma
【FEATURES】
Viral RNA&DNA Extraction Kit (Spin column) The kit utilizes
silica-based membrane
technology in the form of a convenient spin column. Viral nucleic
acids from lysed
samples bind to the column membrane while impurities are
effectively removed
during subsequent washing and centrifugation steps. Ready-to-use
nucleic acids
are eluted from the column. The kit allows simplified isolation of
viral RNA from
cell-free body fluids with a fast spin-column format. No
phenol/chloroform extraction
is required. Viral RNA binds specifically to the silica membrane
while contaminants
are removed in the flow-through. PCR inhibitors such as divalent
cations and proteins
are completely removed in two efficient wash steps, leaving pure
viral RNA to be
eluted in RNase-free water. Purified RNA is ready to use in RT-PCR,
Northern
blotting or other downstream applications.
• The operation process is simple, quick, and non-toxic
• High purity DNA/RNA, stable quality
Benefits
- Comprehensive sample type
Highly-pure viral RNA extraction from various clinical samples such
as serum,
blood samples, CSF (Cerebrospinal Fluid), urine, swab, etc.
- Poly(A) powder
Maximized RNA degradation protection and binding efficiency - High binding capacity
Utilization of Silica Magnetic Particles developed and produced by
proprietary
technology
- Convenience
Ethanol already added to the Wash Buffer
Application
- cDNA synthesis
- RT-PCR
- Quantative real-time RT-PCR
- poly A+ RNA selection
- Northern blot analysis
- Pathogen detection
【PACKAGE SPECIFICATION】
50 tests/kit
【INTENDED USE】
This kit is used to extract DNA/RNA from plasma, serum, animal
tissues, body fluids
and other samples. The sample is processed by the lysis solution to
release DNA/RNA.
At the same time, a special polymer membrane material is used to
selectively adsorb
viral DNA/RNA, and then after washing and elution operations,
high-purity viral
DNA/RNA can be obtained. The nucleic acid extracted by this kit can
be used for
Real-time PCR, Nothern Blot, Blotting Hybridization, cDNA Library
Construction.
The kit can be used for both research purposes and in vitro
diagnosis (IVD) and
is validated for COVID-19 diagnosis.
【COMPONENTS OF THE KIT】
| No. | Reagent Name | Spec. & Qty |
| 1 | Lysis Solution | 15mlx 1 Bottle |
| 2 | Wash Buffer I | 8ml×1 Bottle (add 24ml absolute ethanol before the first use) |
| 3 | Wash BufferⅡ | 8ml×1 Bottle (add 24ml absolute ethanol before the first use) |
| 4 | Elution Solution | 3mlx 1 Bottle |
| 5 | Adsorption Column | 50×1 Pack |
| 6 | Collection Tube | 50×1 Pack |
【STORAGEAND STABILITY】
The kit should be stored at room temperature 5-25℃. The kit is
valid for 12 months.
【Self Provide Materials】
Sterilized 1.5ml centrifuge tube, Sterilized tips of various
specifications, centrifuge,
absolute ethanol, vortex mixer, etc.
【SPECIMEN REQUIREMENTS】
1. The virus in liquid samples such as whole blood, plasma, serum,
ascites, oral fluid
can be directly extracted.
2. The virus in animal and plant tissues needs to add an
appropriate amount of saline
or PBS to the sample, grind it thoroughly, centrifuge at 12000rpm
for 5-10 minutes,
and take the Supernatant for use
【SPECIMEN EXTRATION OPERATION】
1. Add 300ul of the processed sample to a 1.5ml clean centrifuge
tube (if less than
300ul, please make up to 300ul with PBS or saline), and then add 300ul of
Lysis
Solution. Cap the tube and vortex for 30 seconds.
2. Incubate it at room temperature for 10 minutes.
3. Add 200ul of absolute ethanol to the centrifuge tube, cap the
tube, and vortex for
15 seconds. Centrifuge briefly to collect the solution on the tube
wall and tube cap.
4. Put the Adsorption Column into the Collection Tube, transfer the
above mixed
solution to the Adsorption Column, centrifuge at 8000rpm for 1
minute, and discard
the solution in the Collection Tube.
5. Add 600ul of Washing Buffer I to the Adsorption Column (confirm
that absolute
ethanol was added to Washing Solution I before use), centrifuge at 8000rpm
for
1 minute, and discard the solution in the Collection Tube.
6. Add 600ul of Washing BufferⅡto the Adsorption Column (confirm
that absolute
ethanol was added to Washing SolutionⅡbefore use), centrifuge at
8000rpm for l
minute, and discard the solution in the Collection Tube.
7. Put the Adsorption Column back into the collection tube and
centrifuge at 8000rpm
for 2 minutes, discard the solution in the Collection Tube.
8. Transfer the Adsorption Column to a new 1.5ml centrifuge tube,
add 50pl of
Elution Solution to the Adsorption Column and incubate at room temperature
for 2 minutes.
9. Centrifuge at 8000rpm for 1 minute and discard the Adsorption
Column.
The solution in the 1.5ml centrifuge tube contains DNA/RNA. The extracted DNA/RNA
can be directly used in Real-time PCR, Nothern Blot, Blotting Hybridization, cDNA
Library Construction and other experiments. If you do not use it immediately,
please store it at-20℃.
【PRODUCT PERFORMANCE INDEX】
1. The purity:The ratio of absorbance OD260/OD280 is between
1.8-2.2.
2. The precision:The coefficient of variation (CV) of the RNA/DNA
content is≤5%.
【SAMPLE REQUIREMENTS】
• Store at 4°C for no more than 72 hours; at -70°C for long term
storage
• Avoid repeated freezing and thawing
• Swab samples should only be collected with synthetic tip swabs
with aluminum or
plastic shafts.
【PRECAUTIONS】
1. Please read this kit operating instructions carefully before
use, and operate carefully
as required.
2. The virus has a strong infection ability, and various defense
measures must be
prepared before operation.
3. Wear clean gloves and masks during operation.
4. Use RNase/DNase-free tips and 1.5ml centrifuge tubes.
5. All centrifugation steps are performed at room temperature
(5-25℃).
6. Frozen samples need to be thawed to room temperature.
7. Need to bring your own absolute ethanol and 75% ethanol,
8. Do not use products beyond the expiration date; do not mix
reagents of different
lot numbers.
【INDEX OF SYMBOL】
【EXPORTER】
Magnus International Limited
F12, New City International Mansion A, 234 Huapao Ave.
Liuyang, Hunan Province 410300 China
Contact: Goodwellmedical@gmail.com
【AUTHORIZED REPRESENTATIVE】
Lotus NL B.V.
Koningin Julianaplein 10, 1e Verd, 2595AA, The Hague, Netherlands.
【FACTORY VIEW】
【FACTORY WORKSHOP】
【PRODUCT AND STANDARDS】
【CERTIFICATIONS】
【FAQ】
1. Q: How about your healthcare and medical product quality?
A: Quality is our top priority. We have a professional quality
control team to ensure the
quality with a high standard and regulated with the pro
certifications. The QC team is
responsible for quality checking in each process and inspects every
batch of our production
and ships them only after QC approval.
2. Q: What is your order MOQ?
A: Please contact customer service, and the price is negotiable for
a large quantity.
3. Q: How about your product packing for shipment or mailing?
A: We prepared a well neutral package for our products. We could
also make OEM packages,
OEM/ODM Custom Package/Multi-packing package/Cartons with well
protective wrapping.
4. Q: What level of your product price?
A: We are the factory and direct manufacturer and offer competitive
prices. We also offer
volume pricing with more benefits depending on your order quantity
level. Please let us know
your ordering volume while you make the inquiry to us to help you
obtain better benefits.
5. Q: What're the price terms?
A: The price is based on the FOB of Hunan, China with an exchange
rate of RMB6.40 for
USD$1.00. If the exchange rate floating sharply lower than 6.3 or higher than
6.5, we should
adjust the price with the real-time exchange rate.
6. Q: What're the payment terms?
A: We accept T/T wire transferring, PayPal, Western Union, etc. 50%
down payment after
signing the contract and rest 50% before shipping confirmation.
7. Q: How could I get some samples?
A: We are able to deduct your samples expense once your orders are
confirmed with us.
The shipping fee will be calculated with the sample volume that you
demand.
8. Q: Could you accept OEM orders?
A: Yes, we could. We have professional designers who can design the
logo or artwork and offer
best solution & styles for your OEM product choices.
9. Q: How long will the shipping or express take?
A: (1) For the international express such as DHL, UPS, FedEx, TNT,
or EMS, etc., it usually
takes 5-7 working days to your location or destination.
(2) If shipping with containers, they will take about 30-60 days to
process depending on
the various ports or door locations.
(3) We will arrange the delivery or shipping of your products
within 1-3 days after full
payment has been confirmed. We may not ship your products out until your
payments arrive
to our bank account successfully.
10. Q: How do you deliver the goods to us?
A: If the order of small volume package, we could deliver by
courier such as DHL, UPS, FedEx,
TNT, EMS. For the large volume order, we could ship with bulk cargo
containers by sea.
We will provide you with cost-saving recommendations for your
product shipping.
11. Q: What is the warranty time.
A: One year for each product.
【SHIPPING】
We are the trusted healthcare and medical supplies from China.
Be free to contact us at any time!
You are very welcome to email us today at:
Goodwellmedical@gmail.com for the inquiry!
We are excited to build a reliable and long-term business with you
at our best!
